Fig. 2: Widespread misclassification of DNA replication states by FACS for the HCT-116 WT, HCT-116 DKO1 and GM12979 cell lines. | Nature Communications

Fig. 2: Widespread misclassification of DNA replication states by FACS for the HCT-116 WT, HCT-116 DKO1 and GM12979 cell lines.

From: Unravelling single-cell DNA replication timing dynamics using machine learning reveals heterogeneity in cancer progression

Fig. 2

Partial discordance for the replication states of single cells between the FACS results and the supervised deep learning method developed here. FACS sorting of G1 cells (upper panels) and of S cells (lower panels) from wild-type (WT; left) and double knock-out of the maintenance DNA methyltransferase DNMT1 and the de novo DNA methyltransferase DNMT3B (DKO1; centre) HCT-116 cell and GM12878 (right) cell samples. Coloured dots indicate the MnM replication state predictions. Source data are provided as a Source Data file.

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