Fig. 2: The detailed interactions between the YchF(H114A) and 50S subunit.
From: Conserved GTPase OLA1 promotes efficient translation on D/E-rich mRNA
a, c Overview of the interactions between YchF(H114A) (red/blue/green) and 50S (uL14: magenta; bL19: yellow; H62: cyan) shown in two representative views. b Magnified view of the region indicated by box in (a). The key residues of YchF and H62 involved in YchF-50S interactions are shown and labeled. d–f, Magnified views of the regions indicated by boxes in (c). The key residues of YchF and uL14, bL19 involved in YchF-50S interactions are shown and labeled. Cryo-EM map densities are shown in gray with the same level. g Sequence logos created in WebLogo shows that lysine and arginine are conserved in the tip of the helical domain among species. A total of 28 OLA1/YchF sequences from different species were used for the alignment (Supplementary Table 2).
