Fig. 6: Model of the OLA1/YchF-mediated translation regulation.
From: Conserved GTPase OLA1 promotes efficient translation on D/E-rich mRNA
a Flowchart for validating the function of OLA1/YchF. D12, twelve consecutive codons that encode Asp amino acids. b, c The violin plot displays the GFP/mCherry values in each cell. The experiment was conducted three times, and one of these repetitions is shown. The median values of the GFP/mCherry ratio are displayed in the figure, with P values calculated using the paired-sample Wilcoxon signed-rank test (two-sided). d Translation stalling happen on the mRNA for a subset of ribosome proteins and translation regulation factors. e OLA1/YchF and other proteins work together to solve the stalling on D/E-rich region and keep the translation at a normal level. f These translation-related proteins regulate the translation on the final mRNA. g Part of the mRNA with D/E-rich region are degraded when stalling can not be solved in the absence of OLA1/YchF. And the mRNA decay changes the amount of the translation-related proteins. h Less amount of the translation-related proteins generate heterogeneous ribosome complex, resulting in a change of translation efficiency on the final mRNA. i Final products further regulate various cell process. Source data are provided as a Source Data file.
