Fig. 4: FIB-SEM imaging and segmentation of the IOR alteration during ageing. | Nature Communications

Fig. 4: FIB-SEM imaging and segmentation of the IOR alteration during ageing.

From: In situ architecture of the intercellular organelle reservoir between epididymal epithelial cells by volume electron microscopy

Fig. 4

a XY orthoslice of the caput epididymis of FIB-SEM showing a decrease in IORs at 21 m compared with the 56 days caput epididymis. Scale bar: 10 μm. b Three-dimensional reconstruction of the FIB-SEM image of the 21-month caput epididymis. Scale bar: 5 μm. Corresponding analysis of IOR numbers (c) (n = 16 cell-pair for 56 days, n = 24 cell-pair for 21 months, ****p < 0.0001), IOR area (d) (****p < 0.0001), frequency distribution of the IOR area (e) (n = 96 for 56 days, n = 64 for 21 months), frequency distribution of the IOR area at 56 days (f) and 21 months (g). Data are presented as mean value ± SEM. Statistical significance between groups was determined via two-tailed unpaired t-test. Exact p values are provided in Source Data. The source data is provided as a source data file.

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