Table 2 Primers used in this study
Primer name | Primer sequence | Function | Reference |
|---|---|---|---|
WITS01 | acgacaccactccacacct | qPCR on WITS | |
WITS02 | acccgcaataccaacaactc | qPCR on WITS | |
WITS11 | atcccacacactcgatctca | qPCR on WITS | |
WITS13 | gctaaagacacccctcactca | qPCR on WITS | |
WITS17 | tcaccagcccaccccctca | qPCR on WITS | |
WITS19 | gcactatccagccccataac | qPCR on WITS | |
WITS21 | acaaccaccgatcactctcc | qPCR on WITS | |
ydgA | GGCTGTCCGCAATGGGTC | qPCR on WITS | |
WISH028 | TTAGCGAGGTTGGCATCTTTATCC | qPCR on WISH | |
WISH031 | AAATCCATACCCGTTCCTCAATCG | qPCR on WISH | |
WISH033 | CAAGATAGTGGAGAGGTGGAATGC | qPCR on WISH | |
WISH038 | CGTGCTTATCTCTGTCTCGTTACC | qPCR on WISH | |
WISH115 | CTTTCCGTCAGAGCATACCTTACC | qPCR on WISH | |
WISH182 | CGCCTACTATGTGTCTGTGTTACC | qPCR on WISH | |
WISH194 | AATCAATACACGACGGACCTTACG | qPCR on WISH | |
WISH205 | GAACGGTAATGGCACTCTGATAGG | qPCR on WISH | |
WISH206 | GGTAGAAATGGACTGCTGGTATCG | qPCR on WISH | |
WISH207 | CAGAATGTGTAACGCCTCCTATCC | qPCR on WISH | |
WISH208 | CAGTCGGAGAAAGAAGGTCATACG | qPCR on WISH | |
WISH209 | GCTACAATCGCCTACCATCTATCG | qPCR on WISH | |
WISH210 | GCCTATCTTATCCACGCAGTATCG | qPCR on WISH | |
WISH212 | GGCTTCCATTACTCGTTTCATTGC | qPCR on WISH | |
WISH213 | TGCGAATAAACTAAGCCCGATACC | qPCR on WISH | |
WISH214 | TCCCTTTATTATGCGTCGGTTAGC | qPCR on WISH | |
WISH215 | CCAAAGCGAGAGAATCAGGTATCC | qPCR on WISH | |
WISH rev | TATGAGGAGAGTAGGAGGCAATGG | qPCR on WISH | |
ugpB_seq_F | GGACGGGATGATGACATGA | Verification of KO | This study |
ugpB_seq_R | CCCCCGCGAACATAAAACG | This study | |
gldA_seq_F | CGGAGAGAATGGTGCGTGAT | Verification of KO | This study |
gldA_seq_R | GCCAGCTTTAAAACGCCGC | This study | |
glpFK_KO_F | CGGAGTTGCCTCCGCACAAAGATTTTGCCGGATGGCGACC ata tga ata tcc tcc tta gtt | KO of glpFK | This study |
glpFK_KO_R | CGCATACCAATAATCATTACATACTCTTCAGGATCCGATT tgt gta ggc tgg agc tgc ttc | This study | |
glpK_seq_F | gatgtgtgtgcggagttgc | Verification of KO | This study |
glpK_seq_R | GATACCTGCGTCGTGGAAGAG | This study | |
glpF_seq_F | TTCATGGCGCGATAACGC | Verification of KO | This study |
glpF_seq_R | gatgtccatgacctcctcgc | This study | |
glpABC_KO_F | GTATGGCTAAATGATAAAAAACGAACTGTGAGGAAAAACA ata tga ata tcc tcc tta gtt | KO of glpABC | This study |
glpABC_KO_R | AGTAGGGTAATGGCGCCGGAGGGGCTCCGGCGCTTTTTAC tgt gta ggc tgg agc tgc ttc | This study | |
glpABC_seq_F | TGGCCGCGATGTTAAGTAAAG | Verification of KO | This study |
glpABC_seq_F | AGTAACGTCGTTATCTGTCATGC | This study | |
glpD_seq_F | CATTTTATCGCCCAGTGTACG | Verification of KO | This study |
glpD_seq_R | GGAAAACTCTCTGGAATAGCG | This study | |
mgsA_KO_F | ttccagtaatctgtagacaggttaactacggaatcgaattatatgaatatcctccttagtt | KO of mgsA | This study |
mgsA_KO_F | cctaaagcgcgggcggtacagcatcccgcccgcgtagcgttgtgtaggctggagctgcttc | This study | |
mgsA_seq_F | cttgatatgagttgtcccagc | Verification of KO | This study |
mgsA_seq_R | gataatccctcgcgctttgtg | This study | |
pitA_ver_F | CTCAAAATGGCGTAACGTCC | Verification of KO | This study |
pitA_ver_R | GATAAGCCTCCGGATGACG | This study | |
GA_PglpT_F | cgtaatacgactcactatagCGGTAACCGCATAACGAG | Gibson Assemblyof pZ8001 | This study |
GA_PglpT_R | aatactacctcctgaattccATTGTAGCCTCCGTGGCC | This study | |
pM965_amp_F | GGATCCCCCGGGCTGCAGGAATTC | This study | |
pM965_amp_R | AGCGGCCGCCACCGCGGT | This study | |
pZ8049_PpstS_F | cacctaaaATGGCGGCAGGGATGGTAC | Gibson Assembly of pZ8049 | This study |
pZ8049_PpstS_R | acaccataCATAATGTCTCCTGCACGGTTTC | This study | |
pZ8049_pZ_F | gagacattatgTATGGTGTTCACACTGGAAG | This study | |
pZ8049_pZ_R | gccgccatTTTAGGTGGCACTTTTCGG | This study | |
pZ8040_ F | gccacctaaaTATGGTGTTCACACTGGAAG | Gibson Assembly of pZ8040 | This study |
pZ8040_ R | gaacaccataTTTAGGTGGCACTTTTCG | This study | |
pZ8046_PgloC_F | cacctaaaAGCTGCTGGCGCTTGGTG | Gibson Assembly of pZ8046 | This study |
pZ8046_PgloC_R | acaccataCATACTGCTCCTTTGATTAAGCCTC | This study | |
pZ8046_BB_F | gagcagtatgTATGGTGTTCACACTGGAAG | This study | |
pZ8046_BB_R | ccagcagctTTTAGGTGGCACTTTTCGG | This study | |
pZ8044_rpsM_F | cacctaaaTCTAGACGATAAAGTAATGACCCG | Gibson Assembly of pZ8044 | This study |
pZ8044_rpsM_R | acaccataCGGGCCACTATGCACTCC | This study | |
pZ8044_BB_F | tggcccgTATGGTGTTCACACTGGAAG | This study | |
pZ8044_BB_R | atcgtctagaTTTAGGTGGCACTTTTCGG | This study | |
pZ8026_glpTcomp_F | cgtaatacgactcactatagCGGTAACCGCATAACGAG | Gibson Assembly of pZ8026 | This study |
pZ8026_ glpTcomp _R | aatactacctcctgaattccTTAGCCTCCGTTGCGTTTTAAC | This study | |
pZ8026_BB_F | GGAATTCAGGAGGTAGTATTG | This study | |
pZ8026_BB_R | CTATAGTGAGTCGTATTACGC | This study |
