Fig. 5: Cells with nonsense mutations in rsbRA and rsbS to eliminate all stressosome components display environmental stress responses.
From: Stressosome-independent but RsbT-dependent environmental stress sensing in Bacillus subtilis
A Schematic of the 5’ portion of the rsbRA operon showing the points at which stop codons were introduced to the rsbRA and rsbS genes at their native chromosomal loci. B Graph showing β-galactosidase activity from PrsbV as a proxy for σB activity (measured at 15-min intervals for 3 h) upon stimulation of the general stress response with 3% ethanol. WT, strain (CSS1113) carrying all four RsbR paralogs. RsbRA only, strain (CSS1480) producing RsbRA as the only RsbR paralog in the cell. No RsbR paralogs, strain (CSS1531) deleted for all four rsbR paralogs. RsbRA only RsbSSTOP, strain (CSS1681) encoding RsbRA as the only RsbR in the cell and bearing an early stop codon in rsbS. C Graph as in B but also showing the response (orange trace) of a strain (CSS1686) with early stop codons in rsbRA and rsbS. D Graph as in C but comparing the listed strains (WT, CSS1113; RsbRA only, CSS1480; No RsbRs, CSS1531; ΔrsbRAΔrsbS, CSS1441; RsbRASTOP RsbSSTOP, CSS1686) with respect to their σB responses to 0.6 M NaCl. All traces are means of biological triplicate experiments, with the shading indicating standard deviation. Source data are provided as a Source Data file.
