Fig. 9: The therapeutic effects of ZNF252P/c-Myc in tumorigenesis. | Nature Communications

Fig. 9: The therapeutic effects of ZNF252P/c-Myc in tumorigenesis.

From: 8q24 derived ZNF252P promotes tumorigenesis by driving phase separation to activate c-Myc mediated feedback loop

Fig. 9

A The immunoblotting assay detected c-Myc expression after treatment with the different concentrations of 10058-F4. Data are representatives of 3 independent experiments. B IC50 assay showed decreased cell viability as 10058-F4 concentration rose. Data are mean ± s.d.; n = 3 independent experiments. C MTT assay detected the effect of indicated treatments on cell proliferation. Data are mean ± s.d.; n = 3 independent experiments, one-way ANOVA with Dunnett’s multiple comparisons test. D The cell xenograft model in nude mice showed the better tumor inhibition effect with the combination of 10058-F4 and ZNF252P ASO (intratumoral injection). Tumor sections were stained with antibodies against Ki-67 and c-Myc. Scale bar 200μm. Data are representatives or mean ± s.d.; n = 5 mice per group, one-way ANOVA with Dunnett’s multiple comparisons test. E The combination of 10058-F4 and ZNF252P ASO reduced the tumor growth in PDX models (PDX 1# and PDX 2#). Tumor sections were stained with antibodies against Ki-67 and c-Myc. Scale bar 200μm. Data are representatives or mean ± s.d.; n = 4 mice per group, one-way ANOVA with Dunnett’s multiple comparisons test. F The schematic model of 8q24-driven ZNF252P in cancer. This diagram was created in BioRender. Zhang, E. (2025) https://BioRender.com/k34r796. Source data are provided as a Source data file.

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