Fig. 1: Cohesin and CTCF bind chromatin increasingly more efficient during zebrafish development. | Nature Communications

Fig. 1: Cohesin and CTCF bind chromatin increasingly more efficient during zebrafish development.

From: Increasingly efficient chromatin binding of cohesin and CTCF supports chromatin architecture formation during zebrafish embryogenesis

Fig. 1

a Scheme of the cohesin complex. b Scheme outlining the workflow of zebrafish measurements. c Top: Scheme of continuous illumination. Bottom: Example single-molecule tracks of the 64-cell stage (bright pink) and shield stage (dark pink). Scale bar: 1 µm. Fractions of a three-component diffusion model fitted to jump distance distributions of (d) HT-Rad21 and (e) HT-CTCF. Colors indicate stages of development (64-, 128-, 256-, 512-, 1k-cell, high, oblong, sphere, shield). Bars represent mean values ± s.d. from 500 resamplings using 80% of randomly selected jump distances. For fits and diffusion coefficients see Supplementary Fig. 6-8 and statistics in Supplementary Table 1. f Scheme of interlaced time-lapse microscopy (ITM). Molecules are classified into different binding classes. Long (green): tracks surviving at least two long dark times (8.2 s). Short (red): Tracks surviving one short dark time (0.2 s). Spot (yellow): Molecules only detected in a single frame. Top: Example nuclei of (g) HT-Rad21 and (h) HT-CTCF ITM movies with tracks colored according to the binding classes in panel (f). Grey tracks survived only one long dark time. Scale bar: 5 µm. Bottom: Fractions of short and long binding events of (g) HT-Rad21 (WT) or HT-Rad21-3x mutant and (h) HT-CTCF (WT) or HT-CTCF-ΔZF4-7 mutant. Data represent mean ± s.d. of movie-wise determined fractions. Insets show zooms into the respective graphs. Lines serve as guides to the eye. Statistics and p-Values are provided in Supplementary Table 2–7. Raw data plots are provided in Supplementary Fig. 9. i Fractions of short (left) and long (right) binding events of HT-Rad21 (WT) in the presence of morpholinos (MO) or RNA-polymerase inhibitors in the shield stage. Data represent mean ± s.d. of movie-wise determined fractions. All developmental stages are shown in Supplementary Fig. 14 and 16. P-values were calculated using two-sided Multiple Mann-Whitney test with a false discovery rate set to 1% (Supplementary Tables 8, 9). Source data are provided as a Source Data file for Fig. 1d, e, g–i.

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