Fig. 8: Disruption of mitochondrial function and mobility impairs ZIKV replication and modulates the IFN response. | Nature Communications

Fig. 8: Disruption of mitochondrial function and mobility impairs ZIKV replication and modulates the IFN response.

From: Zika virus NS1 drives tunneling nanotube formation for mitochondrial transfer and stealth transmission in trophoblasts

Fig. 8

A Rotenone is a reversible mitochondrial electron transport chain complex I inhibitor. B Cytotoxicity of rotenone in JEG-3, n = 4, 48 hrs. C Rotenone inhibits ZIKV growth in JEG-3 infected cells (MOI = 0.1, 48 hrs, n = 3) as determined by plaque assay. D Miro1 reducer induces proteasomal degradation of MIRO1. E Cytotoxicity of Miro1 reducer in JEG-3 cells, n = 4, 48 hrs. F Miro1 reducer inhibits ZIKV growth in JEG-3 infected cells (MOI = 0.1, 48 hrs, n = 3) as determined by plaque assay. G-K IFN levels in ZIKV infected (MOI = 0.1, 48 hrs, n = 4) JEG-3 cells treated with Rotenone (0.01 μM; DMSO 0.0001%) and Miro1 reducer (40 μM; DMSO 0.4%). Secreted IFN levels in ZIKV-infected cells treated with rotenone or Miro1 reducer are expressed in pg/mL. Data are presented as median and interquartile range (G, H) and mean ± SEM (B, C, E, F, I, J, K). Statistical significance was determined by ANOVA followed by Dunnett’s multiple comparison tests (B, C, E, F, I, J, K) and Kruskal-Wallis followed by Dunn’s multiple comparison tests (G, H). *= P ≤ 0.05, ***= P ≤ 0.001, and ****= P ≤ 0.0001. Figures A and D were created in BioRender.129. Source data are provided as a Source Data file.

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