Fig. 2: Activation of Cph1-PM augments membrane tension, ERM, and focal adhesion expression.
From: Intercellular adhesion boots collective cell migration through elevated membrane tension
Pro12A staining shows polarization changes on changing illumination (a) from far-red to red light and (b) from red to far-red light. Ordered phase images were used to detect the cell boundaries. Plots depicting the normalized general polarization at the cell-cell interface on changes from far-red to red light (a; n = 8) or from red to far-red light (b; n = 8) (n = 3 biological replicates; two-tailed, paired sample t-test). c Schematic showing how the static tether pulling force is measured by atomic force microscopy (AFM). d Static tether force of Cph1-PM-MDA cells exposed to far-red (n = 20) or red (n = 20) light (3 biological replicates, two-tailed, two-sample t-test). e Schematic representation of phosphorylation of Ezrin/Radixin/Moesin proteins (pERM) proteins. f Confocal fluorescence images of Cph1-PM-MDA cells stained with phalloidin (cyan), anti-pERM antibody (yellow), and DAPI (violet). g Quantification of pERM area per cell under far-red (n = 32) and red (n = 32) light exposure (3 biological replicates, two-tailed, two-sample Mann-Whitney test). h Confocal fluorescence images of Cph1-PM-MDA cells stained with phalloidin (cyan), anti-vinculin antibody (yellow), and DAPI (violet). i Cell spreading area (two-tailed, two-sample Mann-Whitney test), j focal adhesion (FA) area per cell (two-tailed, two-sample Mann-Whitney test), k total FA area per cell (two-tailed, two-sample Mann-Whitney test), and l number of FAs per cell (two-tailed, two-sample t-test) of Cph1-PM-MDA cells exposed to either red (n = 69) or far-red (n = 67) light from 3 biological replicates. Bar plots (a, b) are reported as the mean and whiskers represent the standard deviation. The box plot (d, g, i, j, k, l) presents the median and the 25th and 75th percentiles. The lower and upper boundaries of the whiskers show the 5th and 95th percentiles. Scale bars (a, b, f, h) are 10 µm. Data source and statistical details are provided as a source data file.
