Fig. 3: Structural comparison and conformational transition.

a, b The solvent accessibility of the urea binding site in the structure of DUR3^urea (a) and DUR3^apo (b). The urea molecule is shown as spheres, and its adjacent residues are shown as sticks. c, d Structural comparison of the urea binding site between DUR3^apo (blue) and DUR3^urea (red), shown in two perpendicular views from the membrane plane. The urea molecule and residues interacting with urea are shown as sticks and labeled. Y315 exhibited notable conformational shifts between the two states and are indicated using black dots and arrows. The two alternative conformations of Y315, Y315^A ([A]) and Y315^B ([B]), are labeled. Putative hydrogen bonds are shown as black dotted lines.