Fig. 2: Microscopic images and a tomographic model of the cell adhesion interface by γB4-ΔIC.

A A schematic diagram of the domain arrangement of γB4-ΔIC is shown on the top, the GFP tag is shown in green. A confocal fluorescent image of an adhesion interface (white arrowheads) by γB4-ΔIC is shown on the left (scale bar, 5 μm). EM images of an adhesion interface (white arrowhead) (scale bar, 1 μm) with a zoom-in view (white arrows) (scale bar, 100 nm) are shown in the middle. A gallery of the γB4-ΔIC mediated adhesion interfaces (white arrows) is shown on the right (scale bar, 50 nm; more than thirty independent interfaces are imaged). B EM images of a γB4-ΔIC mediated adhesion interface visualized at different tilt angles (scale bar, 100 nm). C A tomographic slice of a γB4-ΔIC mediated adhesion interface (scale bar, 35 nm). D A segmentation model of the tomogram of the γB4-ΔIC mediated adhesion interface shown in (C). The cell membranes and the densities in between are colored pink and green, respectively. E The trans- and cis-dimers and tetramer of γB4 ectodomain found in crystals. The monomers are colored light green, green, cyan, or blue. F The segmentation model shown in (D, white rectangle) is fitted with the trans-dimers of the ectodomain of γB4 (green or cyan) (left), revealing the assembly pattern of γB4-ΔIC between cell membranes (right). G A 3D tomographic model of the assembly of γB4-ΔIC at the adhesion interfaces. The cell membranes are colored gray. γB4-ΔIC are shown in yellow, blue, or red.