Fig. 4: Microscopic images of the cell adhesion interfaces by the EC5 mutants of γB4-ΔIC.
From: Structural insights into the in situ assembly of clustered protocadherin γB4
A A schematic diagram of γB4-ΔIC is shown on the top. The sequence alignment of EC5 from γB4 and γB6 is shown at the bottom. The residue differences between γB4 and γB6 on EC5 are labeled in green, yellow, red, or purple. B A confocal fluorescent image of an adhesion interface (white arrowheads) by the mutant T451Q/V453S is shown on the left. An EM image of an adhesion interface (white arrows) is shown in the middle. A gallery of the mutant-mediated adhesion interfaces (white arrows) is shown on the right (more than ten independent interfaces are imaged). C A confocal fluorescent image of an adhesion interface (white arrowheads) by the mutant Q484H/Y488S is shown on the left. An EM image of an adhesion interface (white arrows) is shown in the middle. A gallery of the mutant-mediated adhesion interfaces (white arrows) is shown on the right (more than eight independent interfaces are imaged). D A confocal fluorescent image of an adhesion interface (white arrowheads) by the mutant E497K is shown on the left. An EM image of an adhesion interface (white arrows) is shown in the middle. A gallery of the mutant-mediated adhesion interfaces (white arrows) is shown on the right (more than thirteen independent interfaces are imaged). E A confocal fluorescent image of an adhesion interface (white arrowheads) by the mutant H535Q/S537K is shown on the left. An EM image of an adhesion interface (white arrows) is shown in the middle. A gallery of the mutant-mediated adhesion interfaces (white arrows) is shown on the right (more than five independent interfaces are imaged). Scale bar, 5 μm (left), 100 nm (middle), 50 nm (right).
