Fig. 5: Cis-dimeric interaction of the in situ assembly of γB4-ΔIC.

A Cis-dimeric interaction of γB4 ectodomain in the crystals. EC5 and EC6 from the two monomers are colored gray or green. The position of E497 from one of the monomers is labeled. B Cis-dimeric interaction of γB4-ΔIC on the cell surface. EC5 and EC6 from the two monomers are colored gray or green. The position of E497 from one of the monomers is labeled. A positively charged region (blue) from EC6 that may be approached by E497 during the in situ assembly of γB4-ΔIC is also shown (dashed rectangles). C The potential cis-dimeric interface of γB4-ΔIC on the cell surface (dashed orange rectangle). The positions of L585 and V590 are labeled. D The fluorescent images of cell adhesion mediated by γB4-ΔIC and γB4 mutants (L585A and V590G). The adhesion interfaces are indicated by white arrowheads (scale bar, 15 μm). E The statistics of the adhesion interfaces by γB4-ΔIC and γB4 mutants (L585A and V590G). Each dot represents the percentage of highlighted fluorescent interfaces that appeared in the pairs of neighboring cells in a stochastic field of view. A total of twenty dots were collected for each construct (five views per experiment and repeated four times). The means of the data are plotted and also provided as a source data file. The GFP-transfected cells are applied as a control.