Fig. 7: Microscopic images and the statistics of the adhesion interfaces by the IC-truncation mutants of γB4.

A A schematic diagram of γB4-ΔCIC is shown on the top. A confocal fluorescent image of an adhesion interface (white arrowhead) by γB4-ΔCIC is shown on the left (scale bar, 5 μm). EM images of an adhesion interface (white arrowhead) (scale bar, 1 μm) with a zoom-in view (white arrows) (scale bar, 100 nm) are shown in the middle. A gallery of the γB4-ΔCIC mediated adhesion interfaces (white arrows) is shown on the right (scale bar, 50 nm; more than six independent interfaces are imaged). B A tomographic slice of a γB4-ΔCIC mediated adhesion interface (left) (scale bar, 35 nm) and a segmentation model of the tomogram (middle). The cell membranes and the densities in between are colored pink and cyan, respectively. The densities are tentatively docked with the trans-dimers of the ectodomain of γB4 (green or cyan) (right). C A schematic diagram of γB4-ΔVIC is shown on the top. A confocal fluorescent image of an adhesion interface (white arrowhead) by γB4-ΔVIC is shown on the left (scale bar, 5 μm). EM images of an adhesion interface (white arrowhead) (scale bar, 1 μm) with a zoom-in view (white arrows) (scale bar, 100 nm) are shown in the middle. A gallery of the γB4-ΔVIC mediated adhesion interfaces (white arrows) is shown on the right (scale bar, 50 nm; more than eleven independent interfaces are imaged). D A tomographic slice of a γB4-ΔVIC mediated adhesion interface (left) (scale bar, 35 nm) and a segmentation model of the tomogram (middle). The cell membranes and the densities in between are colored pink and orange, respectively. The densities are tentatively docked with the trans-dimers of the ectodomain of γB4 (green or cyan) (right). E Statistics of the intermembrane distances of the in situ assemblies of γB4-FL, γB4-ΔCIC, γB4-ΔVIC, and γB4-ΔIC. F The statistics of the adhesion interfaces by γB4-FL, γB4-ΔCIC, γB4-ΔVIC, and γB4-ΔIC. Each dot represents the percentage of highlighted fluorescent interfaces that appeared in the pairs of neighboring cells in a stochastic field of view. A total of twenty dots were collected for each construct (five views per experiment and repeated four times). The means of the data are plotted and also provided as a source data file. The GFP-transfected cells are applied as a control. The data for γB4-ΔIC and GFP are also shown in Fig. 5E.