Fig. 5: Investigation of synaptic properties in control- and patient-derived neuronal cultures.
From: Neuronal hyperactivity in neurons derived from individuals with gray matter heterotopia
a Micrographs of 10 weeks old control- and patient-derived 2D neurons immunostained for MAP2 and SYN1/2. b Quantification of the number of SYN1/2 puncta related to the MAP2 process area of control- and patient-derived neurons. c Micrographs of sections of 9 months old control- and patient-derived hCOs immunostained for MAP2 and SYN1/2. d Quantification of the intensity of SYN1/2 puncta related to the MAP2 process area of control- and patient-derived neurons. e Representative mEPSC recording traces obtained by whole-cell voltage-clamp measurements. Quantification of mEPSC frequency (f) and amplitude (g). h Scheme of isolation of synaptosomes enriched fractions from hCOs. SV (synaptic vescicles), Mito (mitochondria), PSD (postsynaptic density). i Graph showing significantly enriched GO terms of the proteome analysis performed on tissue isolated from hCOs, fractions enriched in synaptosomes. GO analyses show enrichment for biological process and cellular component of proteins upregulated in DCHS1 synaptosomes (Supplementary data 1). Scale bars: 10 µm (a), 100 µm (c). Data are represented as mean ± SEM. Statistical significance was based on one-way ANOVA with Turkey’s multiple comparison tests (b, d, f, g) and Fisher’s Exact (i) (*P < 0.05, ***P < 0.001, ****P < 0.0001). Independent wells (b, f, g) or hCOs (d) were analyzed. Every dot in the plots refers to independently analyzed neuronal processes (b, d) or neurons (f, g). At least twentyfive (n = 25) randomly chosen neuronal processes or eleven (n = 11) neurons were analyzed across three (N = 3) (b, d) or two (N = 2) (f, g) independent batches. Source data are provided as a Source Data file, including the exact p values and n numbers. Created in BioRender. Di Matteo, F. (2025) https://BioRender.com/m95d251; https://BioRender.com/y48a635.
