Fig. 2: TF expression is enhanced in the colon and on infiltrating Teffector cells during T cell transfer-induced colitis. | Nature Communications

Fig. 2: TF expression is enhanced in the colon and on infiltrating Teffector cells during T cell transfer-induced colitis.

From: Tissue factor-dependent colitogenic CD4+ T cell thrombogenicity is regulated by activated protein C signalling

Fig. 2

a Schematic diagram of the T cell transfer model of colitis. CD4+ T effector cells were isolated from donor C57BL/6 wild-type (WT) mice and i.p. injected into host Rag1−/− mice (Rag1−/− T cells). Rag1−/− mice i.p. injected with PBS were used as a control (Rag1−/− vehicle). b Colitis developed over a 4-week period, and disease progression was measured by % weight loss compared to original weight. c This was confirmed by subsequent colon histology analysis. d TF staining and e Corrected total fluorescence (CTF) of mice colons following T cell transfer-induced colitis. f TF and CD3 co-staining of mouse colons following T cell transfer-induced colitis (reproduced n = 4 Rag1−/− T cells and n = 3 Rag1−/− vehicle). 2-way ANOVA (b) or two-tailed Student’s t-test (c, e) was used to determine statistical significance from 3–4 biological replicates, n = 4 male Rag1−/− T cells and n = 3 male Rag1−/− vehicle, expressed as mean ± s.e.m. Scale bars: 100 µm (d, f). Source data are provided in the Source Data file. Created in BioRender. Preston, R. (2025) https://BioRender.com/o91u731.

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