Fig. 4: CD4+ T cell activation upregulates markers of TF decryption. | Nature Communications

Fig. 4: CD4+ T cell activation upregulates markers of TF decryption.

From: Tissue factor-dependent colitogenic CD4+ T cell thrombogenicity is regulated by activated protein C signalling

Fig. 4

CD4+ T cells were isolated from donor human blood and skewed to θ (unactivated cells), Th0, and Th1 cell subtypes as described previously. a ASMase translocation to the cell surface, d PS exposure on the outer membrane leaflet, and g PDI recruitment to the cell surface were assessed. TF decryption pathways in θ, Th0 and Th1 were analysed by b, c ASMase cell surface expression, e, f fluorescently labelled lactadherin binding to exposed cell surface PS, and h, i cell surface PDI expression. Student’s paired t-test (two-tailed) (c, f, i) was used to determine statistical significance. Data is expressed as mean ± s.d. (c, f, i) for 5 (c), 8 (f), and 4 (i) biological donors/group. Source data are provided in the Source Data file. Created in BioRender. Preston, R. (2025) https://BioRender.com/x11c079.

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