Fig. 7: Activated PC inhibits TF-mediated T cell thrombogenicity in IBD patient peripheral CD4⁺ T cells.

CD4⁺ T cells were isolated from donor adult human peripheral blood, inflamed non-IBD paediatric peripheral blood and paediatric IBD peripheral blood, plated at a density of 1 × 10⁶/ml and incubated at 37 °C in AIM-V media supplemented with immune replacement serum, +/- 20 nM of activated PC for 24–48 h. a–d Cells were washed in EDTA-containing PBS, and their ability to initiate thrombin generation was analysed by calibrated automated thrombinography in FXII-deficient plasma. b Lagtime, c ETP and d peak thrombin levels were measured and compared, as was their ability to facilitate FXa generation (e). Mann–Whitney U Test (two-tailed) (b–d) or Student’s t-test (two-tailed) (e) was used to determine statistical significance for all conditions except IBD vs IBD + activated PC. For these conditions, either the Wilcoxon test (two-tailed) (b–d) or the Student’s paired t-test (two-tailed) (e) was used as the data is matched. Data is expressed as mean ± s.e.m. for b–d 21 biological donors (n = 13 IBD/IBD activated PC, n = 2 inflamed non-IBD, N = 6 healthy adult) and e 19 biological donors (n = 13 IBD, n = 2 inflamed non-IBD, N = 4 healthy adult). Source data are provided in the Source Data file.