Fig. 5: Mo-AM TREM2 inhibits ATII regeneration and differentiation into ATI.
From: TREM2 promotes lung fibrosis via controlling alveolar macrophage survival and pro-fibrotic activity
A, B TREM2 (+/+) and TREM2 (-/-) Mo-AMs were isolated by flow sorting from mice treated with i.t. BLM for 1 week, respectively. The AMs were mixed with sorted ATIIs (5:1 ratio) in Matrigel and cultured for 12 days. ATII organoid number and size in each group were determined by ImageJ. n = 12 independent culture of cells per group; mean ± SD; unpaired two-tailed Student’s t-test. Scale bar: 2 mm. C–E ATII organoids from “A” were harvested and frozen sections prepared. Immunofluorescence microscopy was performed for the indicated proteins. Scale bar: 20 µm. All experiments were repeated twice with similar results obtained. Source data are provided as a Source Data file.
