Fig. 1: Acyltransferases Mac1 and Mac3 enrich in peroxisome subpopulations and peroxisomal subdomains.

U. maydis strains expressing N-terminally GFP-tagged versions of Mac1 and Mac3 (cyan) and the peroxisomal marker protein mCherry-SKL (magenta) were inspected by epifluorescence microscopy. Representative images of cells grown in glucose (a) or oleate (b). Full images are shown as overlays of two channels. For insets single channels and merged channels are provided. Scale bars: 5 µm. c Quantifications show Pearson’s correlation coefficients of GFP and mCherry signals for indicated strains. Each dot represents one biological repilcate. Center line, mean; error bars, standard error of the mean. Statistical tests were performed by GraphPad Prism via a 1-way Anova combined with a Tukeys post-test to assess significance of the differences. * refers to a p value of 0.0403; ns, not significant p value: 0.2013. Source data are provided as a Source data file. d Cells incubated in medium lacking nitrogen to induce glycolipid biosynthesis were imaged 4 h after inoculation. GFP-Mac1 was expressed under control of its endogenous promoter and the fluorescence signal is depicted in cyan. For insets single channels and merged channels are shown. mCherry-SKL, magenta. Scale bars: 5 µm. e Cells were analyzed by super-resolution microscopy using SIM. Representative images of cells expressing GFP-Mac1 (cyan) and mCherry-SKL (magenta) (left panel), GFP-Mac3 and mCherry-SKL (middle panel), and GFP-SKL and mCherry-SKL (right panel). Full images are shown as overlays of two channels. For insets single channels and merged channels are depicted. Scale bars: 5 µm. f 3D-reconstruction (x,y) of GFP-Mac3 and GFP-SKL (cyan) containing peroxisomes, mCherry-SKL (magenta). Scale bars: 0.5 µm.