Fig. 6: Conservation of core formation properties.
From: Peroxisomal core structures segregate diverse metabolic pathways
a Mus musculus urate oxidase (MmUOX1) was tagged with GFP (cyan) and expressed in U. maydis cells either co-expressing mCherry-SKL (magenta) or mCherry-Mac3 (magenta). b Crude organelle preparations of indicated strains were imaged by epifluorescence microscopy after incubation in lysis buffer supplemented with Triton X-100 (right). Preparations incubated in lysis buffer without Triton X-100 served as control (left). c Constructs expressing GFP-TIIV-SKL and mCherry-SKL were transfected into RPE1 cells. Cells were analyzed by SIM. The representative picture shows an overlay of the GFP-signal (cyan) and the mCherry-signal (magenta) in the overview. The signal of single channels as well as the overlay are depicted for magnified insets. d M. musculus urate oxidase (MmUox1) was tagged with mCherry (magenta) and co-expressed with GFP-TIIV-SKL (cyan) in RPE1 cells. Cells were analyzed by SIM. Representative pictures are organized as above. Scale bars: 5 µm.
