Fig. 5: scRNAseq - hierarchical cluster and detailed analysis of individual lymphocyte clusters with significant differences between the BCG- and ΔsigH-vaccinated groups.
a Hierarchical clustering of top genes from the nine clusters identified as lymphocytes. b–g Bubble plots highlighting six key lymphocyte clusters (C0, C1, C2, C6, C7, C9) show the expression of selected significant markers within each cluster, illustrating differences in expression levels between the BCG (
) or ΔsigH (
)—vaccination groups three weeks post-vaccination. The color of each dot indicates the normalized expression level, while the dot size represents the percentage of cells within that cluster expressing the corresponding gene. To identify cluster-specific markers, we compared the gene expressions of each cluster against all other clusters combined using Wilcoxon rank-sum tests, with Bonferroni correction for multiple hypothesis testing with adjusted p-value threshold of 0.05.
