Fig. 3: SAR247799 maintains endothelial barrier in colitis mice. | Nature Communications

Fig. 3: SAR247799 maintains endothelial barrier in colitis mice.

From: S1PR1-biased activation drives the resolution of endothelial dysfunction-associated inflammatory diseases by maintaining endothelial integrity

Fig. 3: SAR247799 maintains endothelial barrier in colitis mice.The alternative text for this image may have been generated using AI.

a mRNA expression of S1pr1 in colon of DSS-induced colitis mouse with SAR247799 and FTY720 treatments, Ctrl: Ctrl + Vehicle (n = 3), DSS: DSS + Vehicle (n = 3), FTY720: DSS + FTY720 (n = 3), SAR: DSS + SAR247799 (n = 3). b mRNA expression of endothelial markers shows a positive correlation (Spearman) with S1pr1 expression (n = 3). c Summary of the count of immune- and endothelial-related pathways in all pairwise comparisons of GOBP enrichment results. d Heatmap illustrating GSVA scores for pathways related to endothelial functions (n = 3). e mMCP scores for the four groups (n = 3). f Heatmap of genes involved in endothelial-related pathways identified through GOBP enrichment analysis (n = 3). g mRNA level of Pecam, Vcam1, Cdh5, Sele and Icam1, (n = 6). h Representative IF for Vcam1(red), CD3 (green) and CD31 (violet) colon of colitis mice(n = 6). Scale bar, 50 μm. Data are presented as mean ± SEM; Statistical analysis: two-sided Student’s t test (a, e, g) and Spearman’s rank correlation analysis (b). All biological experiments were repeated three times and yielded consistent results. Source data are provided as a Source Data file.

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