Fig. 7: Biased SAR247799 S1PR1-Gi activation is essential for endothelial barrier therapy.
a DSS-induced Tox1ΔCdh5mice and Arrb2ΔCdh5 mice colitis with SAR247799(1 mg/kg) and FTY720 (0.3 mg/kg) treatments (intragastric gavage, i.g.) body weight percentage (n = 6). b disease activity index (n = 6). c Representative images of colon (left) and quantification of colon length (right) (n = 6). d Representative coloscopy images (top) and MEICS scores (bottom). e Representative images of H&E (left) and HAI analysis (right) (n = 6). f Quantification of Evans blue dye leakage from blood vessels (n = 6). Data are presented as mean ± SEM; Statistical analysis: Two-way ANOVA with multiple comparisons (a, b), two-sided Student’s t test (c–f). All biological experiments were repeated three times and yielded consistent results.
