Fig. 1: PGLYRP1 specifically binds GMTriP-K.

a Peptidoglycan fragment library: GMTriP-K (1) and MDP (2); synthetic peptidoglycan fragments (1a-b, 2a-h and 3a-d) were all prepared with amine linkage points for attachment to the array surface via NHS-chemistry. b General workflow for the printing, incubation, and analysis of the PGN small fragment microarray (Created in BioRender. Putnik, R. (2025) https://BioRender.com/x50o125). c Microarray binding of PGLYRPs. PGLYRP1 binds to the disaccharide components of the array, whereas PGLYRP3 and PGLYRP4 show no association with the compounds on the array. d Determination of Apparent Dissociation Constant for PGLYRP1 to GMTriP-K (3 independent experiments were carried out with each experiment done in technical replicates). e Alphafold protein structure prediction of human PGLYRP1. f–i Docking prediction of GMTriP-K to human PGLYRP1. j Docking-prediction of PGLYRP3 and GMTriP-K demonstrating shallow GlcNAc preventing interaction. For synthetic procedures and compound characterization (NMRs, HRMS), please see the SI. For (c), each condition was screened in at least biological triplicate and technical replicated (see SI for raw image files and other biologically replicate binding data). Data are presented as mean ± SEM. All experiments were repeated twice and yielded consistent results.