Fig. 9: Cell-binding by POGase.
From: Dual functional POGases from bacteria encompassing broader O-glycanase and adhesin activities
Wild-type HEK293 cell line (HEK293-WT), and its isogenic line with Cosmc-mutation (HEK293-CKO) were cultured on 4-well chambered slides for 24 h, and then desialylated with neuraminidase (+neu), fixed, stained with a PNA and anti-Tn mAb; b, c with recombinant 6xHis-tagged POGase TB-FL, -ND, and -CD at 50 µg/mL and corresponding fluorescently labeled streptavidin and secondary antibodies, respectively. DAPI was used to stain nuclei. Images were collected by the fluorescence microscopy at 20× objective. Representative images were shown (n = 4). Bars, 20 μm. The experiment was repeated by immunofluorescence and flow cytometry analyses of a different set of cell lines independently with similar results.
