Fig. 6: Decrease in H3K23ac in Kat6a–/– cells restored to normal in Kat6a–/–Tg(Kat6b) cells. | Nature Communications

Fig. 6: Decrease in H3K23ac in Kat6a–/– cells restored to normal in Kat6a–/–Tg(Kat6b) cells.

From: KAT6B overexpression rescues embryonic lethality in homozygous null KAT6A mice restoring vitality and normal lifespan

Fig. 6

a–g CUT&Tag results detecting histone H3 lysine 23 acetylation in (H3K23ac) in primary mouse embryonic fibroblasts isolated from E14.5 Kat6a+/+Kat6b+/+, Kat6a–/–Kat6b+/+, Kat6a–/–Tg(Kat6b) and Kat6a+/+Tg(Kat6b) E10.5 foetuses. N = 4 foetuses per genotype. Data were analysed as described in the ‘methods’ section. A false discovery rate (FDR) of less than 0.05 was considered significant. a Venn diagram showing number of genes with reduced H3K23ac levels in the indicated samples compared to wild type controls. In comparison to wild type cells Kat6a–/– cells show a global reduction in H3K23ac which is restored to normal in Kat6a–/–Tg(Kat6b) cells. b–d Log2 fold changes in H3K23ac levels at HOX genes (b), TBX genes (c) and DLX genes (e). Note that the reduction in H3K23ac in Kat6a–/– MEFs is restored to normal in Kat6a–/–Tg(Kat6b) MEFs. The FDR is indicated above or below each bar. e–g Read depth plots of H3K23ac in the HOXA gene cluster (e), at the Dlx1/Dlx2 locus (f) and at the Tbx3/Tbx5 locus. Note that the characteristic distribution of H3K23ac in wild type cells is restored in Kat6a–/–Tg(Kat6b) cells.

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