Fig. 2: Dynamics of cytoneme in abdominal histoblasts under EGFR mutant conditions.

A Images from movies of cytonemes protruding from abdominal histoblasts of the P compartment cells expressing UAS.GMA^GFP alone or expressing UAS.GMA^GFP and UAS.EGFR^RNAi at the same time for 20 h in UAS.RHGmiRNA; hh.Gal4 tub.Gal80^ts flies (see Supplementary Movie 1). Scale bars = 10 μm. B Violin plots for lifetimes of cytoneme protruding from abdominal histoblasts in the same mutant conditions as in (C). Note that cytonemes after UAS.EGFR^RNAi expression has shorter lifetimes, indicating that they are more dynamic. C) Images from Supplementary Movie 2 of cytonemes protruding from abdominal histoblasts under hh.Gal4 expressing either GMA^GFP alone, or coexpressing UAS.Ihog^RFP or UAS.Ihog^RFP and UAS.EGFR^RNAi plus UAS.RHGmiRNA at the same time to inhibit apoptosis, (see Supplementary Movie 2). Scale bars = 10 μm. D Violin plots showing cytoneme lifetime in the abdominal histoblasts visualized in (C). Note that Ihog^RFP fails to stabilize cytonemes under EGFR downregulation conditions. Source data are provided as a Source Data file.