Fig. 7: Ihog proteome reveals its interaction with membrane and cytoskeleton regulators.

A A list of proteins specifically isolated from Ihog^RFP affinity purification, with known protein functions relating to cytoskeleton and vesicular trafficking. B–E Cytonemes stabilized by Ihog^RFP expression decrease after transiently inhibiting the actin regulator Cher ((C) compared with the control (B)). D Boxplots showing the quantification of the maximum distance reached by cytonemes within a window measuring the proportional 20% of the wing pouch size to normalize. The statistical analysis performed using two sided pairwise comparison with Wilcoxon tests showed a significant effect of treatment as length decreased after Cher^RNAi expressions (p < 0.0001). E Cytoneme number shows a more moderate reduction after inhibition of Cher (p < 0.0001). F, G Basal sections of Cher^YFP (red) wing discs expressing at endogenous levels and expressing UAS.Ihog^CFP (green) in the P compartment. Note the stabilization of cytonemes induced by Ihog^CFP and the colocalization of Ihog and Cher^YFP in cytonemes (F1, F2). G Quantifications of basal mean gray values of the dorso/ventral ratio of Cher^YFP were analysed using two sided pairwise comparison with Wilcoxon tests (p < 0.002). The panels display the data point distribution with mean and SD whiskers. Note also the increased levels of Cher^YFP in the P compartment, probably due to the recruitment by ectopic Ihog (G2). H Basal section of a Cher^YFP wing disc expressing UAS.Ihog^RNAi in the dorsal compartment. Note the downregulation of Cher^YFP in the absence of Ihog. I Quantifications analysed by two-sided pairwise comparison with Wilcoxon test showing the decrease of the Cher^YFP after Ihog^RNAi expression in the dorsal compartment (p < 0.0002). J Co-immunoprecipitation experiment of larvae extracts expressing UAS.Ihog^RFP and endogenous Cher^YFP, using α-RFP antibody for immunoprecipitation and α-GFP antibody for western blot development, while no protein is observed in the extracts expressing RFP alone as negative control (NC). Scale bars = 10 μm. Source data are provided as a Source Data file.