Fig. 3: Comparison of the Rci51-5/multi-sfxa101 system and Cre/multi-loxP system. | Nature Communications

Fig. 3: Comparison of the Rci51-5/multi-sfxa101 system and Cre/multi-loxP system.

From: Creation of a eukaryotic multiplexed site-specific inversion system and its application for metabolic engineering

Fig. 3

a Two circuits design to validate the recombination outcome of different systems. The 3’ and 5’ of the circuit consist of the promoter array and exogenous genes, respectively. The recombination site is inserted into 3’ of each promoter. The promoter array encompasses a range of promoters with different strengths (color scale): strong promoters pGMP1, pTDH3, pTEF1, medium-strength promoters pPGK1, pPDC1, pTEF2, pTPI1, and weak promoters pCHO1, pADH1, pCYC1. The yellow triangles represent the sfxa101 site, while the green triangles represent the loxP site. b Yeast colonies induced by different recombination systems. The magnified region shows different colony colors and the white colonies of the Rci51-5/multi-sfxa101 system are significantly lower than the Cre/multi-loxP system. The cultures induced by 24 h were spread on synthetic complete medium lacking leucine and uracil (SC-Leu-Ura). The pictures were taken after 7 days incubation at 30 °C. c The proportion of white colonies to total colonies at different induction times. Bars and error bars represent average and standard deviation of three biological replicates, respectively. Source data for this figure are provided as a Source Data file.

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