Fig. 5: Rearrangements in NiV L-P complex upon RNA binding.

a Structural and schematic comparison of the priming and intrusion loops in the apo- and elongating NiV L-P complex structures. Both loops become partially ordered in the elongating NiV L-P complex with an RNA duplex bound. b Detailed view of the interactions between the intrusion and priming loops and other elements in the elongating NiV L-P complex. Key interacting side and main chains are depicted as sticks, with hydrogen bonds highlighted by yellow dashed lines. c Structural overlay and schematic comparison of the supporting helix and loop in the apo (gray) and elongating NiV L-P complex (colored). The supporting loop and helix become ordered in the elongating RNA-bound NiV L-P complex. Interactions involving the supporting helix and loop are shown as yellow dashed lines. d Cartoon representation of the RdRp and CD domains in the elongating NiV L-P complex, with a close-up view of the interactions between them. Hydrogen bonds are depicted as yellow dashed lines and interacting residues are shown as sticks.