Fig. 1: Cryo-EM structure of EBOV nucleocapsid-like structure (NCLS) in VLPs.
From: Structural basis for Ebola virus nucleocapsid assembly and function regulated by VP24
a Helical reconstruction of the NCLS complex at 7.5 Å resolution, with RNA colored in red, nucleoproteins (NP-1 and NP-2) in pink and beige, and viral proteins VP24-1 and VP24-2 in lime green and violet. b Overlay of the cryo-electron tomography (cryo-ET) map of the VLP (virus-like particle) (EMD-3871 [https://www.ebi.ac.uk/emdb/EMD-3871]) and the authentic virus (EMD-3873 [https://www.ebi.ac.uk/emdb/EMD-3873]) compared with the cryo-EM map obtained in this study, showing structural congruence. c Symmetry-expanded and focus-refined cryo-EM map at 4.6 Å resolution showing the arrangement of NPs and VP24 molecules, with VP24-1 in lime green and VP24-2 in violet alongside NPs (NP-1 in pink and NP-2 in beige). Adjacent repeating units are shown in light gray; areas, where the model could not be assigned, are shown in dark gray. d Atomic model of the NCLS. The color scheme for NPs and VP24s matches that of Fig. 1a. e Comparison of the NP structures derived from this study with the previously published purified NP structure (PDB ID: 5Z9W), shown in orange. The left image shows a side view, while the inset displays a 90° rotated top view with the cryo-EM map from this study. The arrow indicates a local structural difference between these NP structures.
