Fig. 2: Action mode of compound Z9 inhibiting TSWV.
a Compound Z9 could inhibit the proliferation of TSWV in tomatoes from the appearance of symptoms. b Schematic diagrams of the constructs SR(+)eGFP and L(+)opt. L(+)opt, a full-length infectious clone of TSWV L genomic RNA fragment with optimized RNA-dependent RNA polymerase (RdRpopt); SR(+)eGFP, an infectious clone of TSWV S genomic RNA with nucleocapsid protein (N), in which NSs of TSWV S genome RNA fragment is replaced by eGFP. Plus sign (+) and 3′ to 5′ designation indicate the antigenomic RNA of TSWV; 2×35S, a double 35S promoter; HH, hammerhead ribozyme; RZ, hepatitis delta virus (HDV) ribozyme; NOS, nopaline synthase terminator. c The amounts of fluorescence in the infiltrated leaves were observed at the 5th day. Bars, 500 µm. The experiment was repeated three times showing similar results. d Western blot was used to detect the accumulation level of protein N in the infiltrated leaves. Staining of RuBisCO with Ponceau S was used as a sample loading control. The experiment was repeated three times showing similar results. e Pattern diagram of continuous detection of binding signal values between small molecules and N protein using BLI. f The binding signal values between compounds with different activities and N protein. Compounds Z1–Z6 are compounds with average activities, while compounds Z8, Z9, Z12, Z13, Z14, and Z16 are compounds with good activity. g The binding ability of compound Z9 with wild-type N protein (NWT) was measured by BLI. Source data are provided as a Source Data file.
