Fig. 2: Development of an intralingual transplantation approach for assessing tissue reconstitution by organoids and stem cells.
From: Microbial metabolites control self-renewal and precancerous progression of human cervical stem cells
a Representative images showing the implantation of human ectocervical organoids in murine tongue tissue. b Representative gross appearance of the organoid-transplanted sites in the murine tongues just after the implantation. c Representative gross appearance of the organoid-transplanted sites in the murine tongues at 4 weeks after implantation. d H&E staining of the human tissues regenerated from the cervical organoids transplanted in the murine tongues displaying a stratified squamous epithelium histology. e Immunofluorescence staining of the human tissues regenerated from cervical organoids with anti-HLA-ABC and anti-KRT14 antibodies. Note the human-derived (HLA-ABC-positive), epithelial (KRT14-positive) tissues formed between the murine tongue musculature. f H&E staining of the human tissues regenerated from ectocervical single cells transplanted in murine tongues, displaying a stratified squamous epithelium histology. g Immunofluorescence staining of the human tissues regenerated from ectocervical single cells with anti-HLA-ABC and anti-KRT14 antibodies. h Frequency of human ectocervical tissue regeneration from human ectocervical organoids or single cells when transplanted inside immunocompromised murine tongues. Tongues were harvested at 3-4 weeks after transplantation. Scale bars: 100 ÎĽm.
