Fig. 2: Niche-specific differential regulation of central metabolic pathways.

A total of 268 differentially expressed genes were identified between isolates harvested from blood and the GI tract, of which 25 corresponded to metabolic genes in the KEGG database. The figure indicates the enzyme, intermediates, and products for each inferred reaction, and enzymes are numbered as defined below. Arrow color defined in key indicates the log₂FC values of differential expression based on group DESeq2 calculations. Enzyme labels are as follows: 1) nitronate monooxygenase, 2) nitrite reductase (NADH) large subunit, 3) nitrite reductase (NADH) small subunit, 4) 4-methoxybenzoate monooxygenase, 5) dihydropyrimidine dehydrogenase subunit A, 6) UDP-N-acetyl-D-glucosamine 6-dehydrogenase, 7) acylamidase, 8) catalase-peroxidase, 9) 3-hydroxycinnamic acid hydroxylase, 10) rhodocoxin reductase, 11) cystathionine beta-lyase, 12) dihydrolipoyl dehydrogenase 13) pyruvate dehydrogenase E2 component, 14) aconitate hydratase, 15) glutamate synthase (NADPH) large chain, 16) glutamate synthase (NADPH) small chain, 17) 4-hydroxy-tetrahydrodipicolinate synthase, 18) diaminobutyrate–2-oxoglutarate transaminase, 19) L-2,4-diaminobutyric acid acetyltransferase, 20) L-ectoine synthase, 21) ectoine hydroxylase, 22) maleamate amidohydrolase, 23) NADH-dependent formate dehydrogenase delta subunit FdsD, 24) aspartyl-tRNA(Asn)/glutamyl-tRNA(Gln) amidotransferase subunit A.