Fig. 6: Small molecule H8 mitigates tissue damage during S. pyogenes infection.

A Structure of H8. B S. pyogenes WT strain was grown in ThyB with or without 20 μg/ml H8, CFU were determined after 18 hrs of incubation under aerobic or anaerobic conditions. C Cultured RAW 264.7 macrophages were infected by WT or ΔPdh in the absence (Ctrl) or presence (+ H8) of 20 μg/ml H8 with net change in CFUs between time 0 and 4 h of infection and (D) concentration of IL-10 in the supernatants determined. E C57BL/6 J mice were subcutaneously infected with 10⁷ CFU WT or ΔPdh, and H8 were injected daily into the site of infection, as shown for determination of (F) Bacterial burden at 3-dpi and (G) Lesion size determined by measuring the area of draining ulcer. H Images from representative ulcers outlined with a red line. Data are pooled from two independent experiments, with each symbol representing an individual mouse (n = 8–10 mice per group, biological replicates). Mean and SEM is shown. Statistical significance was assessed using a two-tailed Student’s t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns: not significant. Exact p-values are provided in the source data. Source data are provided as a Source Data file.