Fig. 6: H2A disordered tails are required for APC8-dependent H2B ubiquitylation.
From: APC/C-mediated ubiquitylation of extranucleosomal histone complexes lacking canonical degrons
a Schematic of APC/C architecture highlighting distance between APC8 and UBE2C active site cysteine, C114. b APC/C-mediated ubiquitylation assays using H2A/H2B dimers with one histone fluorescently labeled and the other histone tailless visualized by fluorescent scanning of SDS-PAGE gels. Representative image of n = 3 experiments. c Ubiquitylation assays of H2B in the context of H2A tail truncations using APC/C platform (Plat), APC Plat + APC8, APC/C or APC/C-CDC20. Representative image of n = 3 experiments. d Model of chromatin-tethered APC/C polyubiquitylating histones mobilized by polymerases or chromatin remodeling complexes, left, or DNA or nucleosome bound proteins, right. Source data are provided as a Source Data file.
