Fig. 4: Tissue-specific loss of imprinting in intra-iDMR patches.
From: Atlas of imprinted and allele-specific DNA methylation in the human body
A Average CpG methylation plot (chr11:2018807-2021899) within the known iDMR for H19/IGF2. The highlighted 692 bp patch (chr11:2020097-2020789) shows monoallelic methylation in purified granulocytes and T cells samples (as expected) but is fully methylated in hepatocytes, and pancreas ductal epithelial samples. An average of 50% methylation is expected for known iDMRs (dotted line). B Sequenced DNA fragments from within the highlighted region, revealing intra-iDMR biallelic methylation. Indeed, almost all fragments from the hepatocytes and pancreas are fully methylated, compared to half of granulocyte and T cell DNA fragments.
