Fig. 2: Longitudinal PET imaging in vaccinated mice.
From: Monitoring mRNA vaccine antigen expression in vivo using PET/CT
a Experimental timeline. Balb/c mice were IM injected with eLNP or S2PΔf-eDHFR mRNA-LNPs in the right hindlimb on day 0. On day 1 and 3 mice were IV administered [18F]FP-TMP and PET/CT images were acquired. b Representative PET/CT images of mice on day 1 and 3. Red arrow indicates [18F]FP-TMP signal in the ipsilateral popliteal LN of the S2PΔf-eDHFR vaccinated mouse on day 1. c [18F]FP-TMP SUVmax quantification in the ipsilateral LN and injected muscle on day 1 (n = 6 mice per group) and day 3 (n = 4 mice per group). d Experimental timeline of follow up experiment. Balb/c mice were vaccinated with S2P or S2PΔf-eDHFR mRNA-LNPs and imaged on day 1. e Representative axial PET/CT slices through the popliteal LNs. Red arrow indicates [18F]FP-TMP signal in the ipsilateral popliteal LN of the S2PΔf-eDHFR vaccinated mouse. f [18F]FP-TMP SUVmax quantification in the ipsilateral LN and injected muscle on day 1 (S2P, n = 4; S2PΔf-eDHFR, n = 6 mice per group). The ALC-307 lipid was used in these experiments. Groups were compared using an unpaired, two-tailed t test. Only significant pairwise comparisons are shown. Data presented as mean ± SD. (c, f) p < 0.0001 unless otherwise stated. day 1: muscle, p = 0.0002. a, d Created in BioRender. Sellmyer, M. (2025) https://BioRender.com/d90y830. Source data for panel (c, f) are provided as a Source Data file.
