Fig. 5: Hybrid ecDNAs interference with each other and reduced after JQ1 treatment. | Nature Communications

Fig. 5: Hybrid ecDNAs interference with each other and reduced after JQ1 treatment.

From: Inhibition of human-HPV hybrid ecDNA enhancers reduces oncogene expression and tumor growth in oropharyngeal cancer

Fig. 5: Hybrid ecDNAs interference with each other and reduced after JQ1 treatment.

Multi-probe FISH, using an EYA2 probe and an HPV probe on hybrid ecDNA on super-resolution DMSO and JQ1 treatment of HMS001 cells, is shown (A and B). Hybrid ecDNAs were observed nearby in the nucleus in the “no treatment” condition (A). Each signal (green: EYA2, red: HPV, and blue: DAPI) was also shown separately (bottom) (A). FISH signals of hybrid ecDNAs were reduced after JQ1 treatment (B). Scale bar shows 5 μm (0.2 μm in the expanded picture). Cartoon illustrating how disruption of interferences between hybrid ecDNAs may decrease transcription is illustrated (right in A and B). Each experiment was repeated twice with similar results.

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