Fig. 1: Interferon (IFN)-γ and C-type lectin (CLEC)-2 are dispensable for thrombosis in the spleen after STm infection.

A Representative immunohistochemistry of spleen sections from WT and IFN-γ-deficient mice (ifng^−/−) infected with STm for one day. Brown = Fibrin, Blue=CD41. B Percentage of section area (left) and number of thrombi per section (right) in spleens from (A); each point represents data from a single mouse, generated from combining results from two independent experiments for a total of n = 7 WT mice and n = 10 IFN-γ-deficient mice. C Representative immunohistochemistry of liver sections from WT and IFN-γ-deficient mice infected with STm for 7 days. Brown= Fibrin, Blue=CD41. D Percentage of section area in livers from (C); each point represents data from a single mouse, generated from combining results from two independent experiments for a total of n = 8 WT mice and n = 8 IFN-γ-deficient mice. E Representative immunohistochemistry of spleen sections from WT and PF4^CreCLEC-2^fl/fl mice infected with STm for one day. Brown = Fibrin, Blue = CD41. F Percentage of section area in spleens from (E); each point represents data from a single mouse, the graph was generated from combining three independent experiments for a total of n = 22 WT mice and n = 12 PF4^CreCLEC-2^fl/fl mice. G Representative immunohistochemistry of spleen sections from WT and PF4^CreCLEC-2^fl/fl mice infected with STm for 7 days. (H) Percentage of section area in spleens from (G); data presented is representative from three independent experiments each with n = 4 mice per group and a single point represents data from a single mouse. Median values shown as horizontal lines on each graph. Error bars depict the 75^th–25^th interquartile range (IQR). Each dot represents an independent mouse. Statistical analyses were performed using the two-tailed Mann–Whitney test. Source data are provided as a Source Data file. ns non-significant, T thrombus, V vessel, RP red pulp, WP white pulp.