Fig. 3: Neutrophils and monocytic cells are required to drive thrombosis in the spleen and liver after STm infection.

A Total numbers of CD11b⁺Gr1^hi cells in isotype control treated (IC) or anti-Ly6G treated mice in the spleen (1 day post-infection) or liver (day 7 post-infection). B Numbers of neutrophils per mm³ in the peripheral blood in isotype control (IC) or anti-Ly6G treated mice that were infected with STm for 1 day (left) or 7 days (right). Representative data from two independent experiments with n = 4 mice per group. C Representative immunohistochemistry sections from spleens (top row; day 1 post-infection) and livers (bottom row; day 7 post-infection) from isotype control (IC) or anti-Ly6G treated mice (Fibrin= brown, CD41=blue). D Quantification of the area occupied by thrombi in spleens and livers from mice represented in (C). Data presented are from two independent experiments combined for a total of n = 8 mice per group. E Representative immunohistochemistry of spleens (day 1 post-infection) and livers (day 7 post-infection) from mice treated with PBS liposomes or clodronate liposomes (Fibrin= brown, CD41=blue). F Quantification of the area occupied by thrombi in the spleens and livers of mice represented in (E). Data presented are from two independent experiments combined for a total of n = 8 mice per group. The data are expressed as dot plots, with each point representing data from a single mouse, and horizontal lines depict the medians. Error bars depict the 75^th–25^th interquartile range (IQR). Statistical analyses were performed using the two-tailed Mann–Whitney test. Source data are provided as a Source Data file. V Vessel, T Thrombus.