Fig. 7: Longitudinal expression of TNF and CD62E in the liver after STm infection.

A Representative images of liver sections from mice infected with 5 × 10⁵ CFU STm SL3261 for 4 h, 8 h, 18 h or 7 days. Sections were stained for TNF (yellow), Ly6G (blue) and F4/80 (red). The bottom row shows the area within the dotted white squares at a higher magnification. Pink arrows show positive TNF staining. B Liver sections from (A) were stained for CD62E (yellow), CD41 (blue) and CD31 (red). The central row shows the area within the dotted white squares at a higher magnification. The bottom row shows CD62E staining only (gray) from the above image. Pink arrows indicate positive staining for CD62E. Non-immunized (NI) n = 4, 4 h n = 4; 8 h n = 3, 18 h n = 3, 7 days n = 4 mice. Representative images from 3-4 mice per group from two independent experiments. NI Non-immunized, V Vessel, T Thrombus.