Fig. 8: STm-induced thrombosis is driven by tissue factor.

A Percentage section area positive for Tissue Factor (TF) in spleens and livers from non-infected controls (NI) or mice infected for 1 day (spleen) or 7 days (liver). Each dot represents a different field of view (FOV) and represents data from a minimum of 10 FOV from each tissue and from a minimum of n = 4 mice for each group. B Representative images of spleen and liver thrombi (from A) stained gray for TF or fibrin (Fib), CD31 (red) and CD41 (blue). C Representative images of spleen and liver thrombi (from A) stained for TF (gray), Ly6G (red) and CD11b (blue). For (B, C) n = 4 spleens and n = 4 livers from four individual mice were assessed from two independent experiments. D Representative images of spleen and liver sections (from A) stained for (gray), α-SMA (red) and CD31 (blue); n = 4 spleens and n = 4 livers from four individual mice were assessed from two independent experiments. E Representative FOV of thrombin activation in the spleen (1 day post-STm infection) and liver (7 days post-STm infection) obtained by intra-vital microscopy. T = 0 min shows the first frame of the recording before the administration of the thrombin probe. T = 5 min shows the frame in the same FOV 5 min after administration of the thrombin probe. Yellow arrows indicate positive thrombin staining. F Quantification of thrombin+ regions 5 min after administration of the thrombin probe in non-infected and infected mice. Data presented are from one experiment with n = 4 mice per group and are representative of at least two experiments for each organ. G Left is a representative image of a splenic thrombus (within the dashed white lines) captured by intra-vital microscopy 24 h after infection, stained for CD49b (red), Ly6G (blue) and F4/80 (gray) next to two images showing thrombin detection (yellow) at t = 0 min and T = 5 min after the administration of the thrombin probe. Images representative of experiments from four infected mice. H Representative sections stained for fibrin (brown) and CD41 (blue) from the spleens (left) and livers (right) of heterozygous mice (expressing human TF and mouse TF, mTF^+/−, hTF^+/−) and mice with low tissue expression (mTF^−/−, hTF^+/+). Data representative of two independent experiments each with n = 4 mice per group. I Quantification of the area occupied by thrombi from G (n = 4 mice per group). Each point represents data from one mouse, horizontal lines depict the medians. Error bars depict the 75^th–25^th interquartile range (IQR). Statistical analyses were performed using the two-tailed Mann-Whitney test. Source data are provided as a Source Data file. T thrombus, V vessel.