Fig. 2: Met- derived metabolite SPD mediates pyroptotic resistance.
From: Methionine metabolite spermidine inhibits tumor pyroptosis by enhancing MYO6-mediated endocytosis
(A) Methionine metabolism schematic. (B) Heatmap of changed metabolites in HONE1 with or without Met deprivation (n  =  4). (C and D) Effects of addition of SAH (10 μM), SPD (50 μM), SPM (50 μM) on Dox-induced PI uptake (C) and LDH release (D) in GSDME-NTTet-On HONE1 and HCT116 starved of Met. (E and F) Effects of DFMO (500 μM) on Dox-induced PI uptake (E) and LDH release (F) in GSDME-NTTet-On HONE1 and HCT116. (G and H) Effects of SRM or AMD1 knockdown on Dox-induced PI uptake (G) and LDH (H) release in GSDME-NTTet-On HONE1 and HCT116. Data are represented as mean ± SD. Two-way ANOVA with Bonferroni’s multiple comparisons test (C–H). NS, not significant. The results are representative of three independent experiments (C–H). Source data are provided as a Source Data file.
