Fig. 5: Application of EcN@INX-2 at the CT26 cells and 3D tumor level. | Nature Communications

Fig. 5: Application of EcN@INX-2 at the CT26 cells and 3D tumor level.

From: Aggregation induced emission luminogen bacteria hybrid bionic robot for multimodal phototheranostics and immunotherapy

Fig. 5: Application of EcN@INX-2 at the CT26 cells and 3D tumor level.The alternative text for this image may have been generated using AI.

a Representative immunofluorescence images showing ecto-CRT (green fluorescence) exposure on the surface of CT26 cells under various treatments. The cell nuclei were stained with DAPI (blue fluorescence). (Scale bar: 20 μm). b Representative immunofluorescence images showing reduced HMGB1 (green fluorescence) in the nucleus of CT26 cells following various treatments. The cell nuclei were stained with DAPI (blue fluorescence). (Scale bar: 20 μm). c CLSM images were captured spanning from the surface to the center of the CT26 tumor spheroid using a Z-stack thickness of 10 μm. The red fluorescence indicates INX-2. (Scale bar: 100 μm). d Histogram depicting the fluorescence intensity profile of a CT26 3D tumor sphere in panel (c) at a depth of 50 μm. e Representative CLSM images showing CT26 3D tumor spheroids co-stained with calcein-AM (green fluorescence) and propidium iodide (red fluorescence) (Scale bar: 100 μm). For a–e, experiment was repeated three times independently with similar results. Source data are provided as a Source Data file.

Back to article page