Fig. 6: Multimodal imaging of EcN@INX-2 in CT26 tumor-bearing female mice.

a Representative NIR-I fluorescence imaging of tumor sites in female mice at different times after intravenous injection of EcN@INX-2 as well as of major tissue organs and tumor tissues isolated at day 10. Yellow circle denote the tumor. b Quantitative analysis of the NIR-I fluorescence intensity of the tumor tissue in panel (a). (n = 3 mice). c Representative NIR-II fluorescence imaging of tumor sites in mice at different times after intravenous injection of EcN@INX-2 as well as of major tissue organs and tumor tissues isolated at day 10. Yellow circle denote the tumor. d Quantitative analysis of the NIR-II fluorescence intensity of the tumor tissue in panel (c) (n = 3 mice). e Representative photothermal imaging of mouse tumor tissue under laser irradiation (660 nm, 0.45 W cm⁻², 10 min) 1 day after tail vein injection of PBS, free INX-2 and EcN@INX-2. f Temperature variations of tumors in panel (e) after exposure to laser irradiation (660 nm, 0.45 W cm⁻², 10 min). (n = 3 mice). g Representative PA images of tumor tissues at 1 day after administration of EcN@INX-2 (After spectral separation, Blue represents deoxyhemoglobin, red represents oxyhemoglobin and green represents INX-2). Data are presented as mean ± SD (n = 3 mice). For (a, c, e and g), experiment was repeated three times independently with similar results. Source data are provided as a Source Data file.