Fig. 3: Subdivision of the CORE cycle into pathway modules facilitates stepwise implementation in E. coli. | Nature Communications

Fig. 3: Subdivision of the CORE cycle into pathway modules facilitates stepwise implementation in E. coli.

From: Design and implementation of aerobic and ambient CO2-reduction as an entry-point for enhanced carbon fixation

Fig. 3: Subdivision of the CORE cycle into pathway modules facilitates stepwise implementation in E. coli.The alternative text for this image may have been generated using AI.

Here, module 1 was established based on endogenous ACC (acetyl-CoA carboxylase) activity and heterologous MCR (malonyl-CoA reductase). Module 2 consists of the BKACE reaction, while implementation of module 3 was pursued based on endogenous activities alone [AtoDA (acetyl-CoA:acetoacetate CoA-transferase); AtoB (thiolase); acetyl-CoA regeneration either via Acs (acetyl-CoA synthetase) or a combination of AckA (acetate kinase) and Pta (phosphotransacetylase)]. The CORE cycle is integrated with subsequent formate assimilation (module 4), which serves as a selectable biosynthesis route to serine and essential one-carbon units of biomass (i.e. formyl-THF, methylene-THF and methyl-THF).

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