Fig. 5: Modules 1, 2 and 4 of the CORE cycle operate robustly together in E. coli. | Nature Communications

Fig. 5: Modules 1, 2 and 4 of the CORE cycle operate robustly together in E. coli.

From: Design and implementation of aerobic and ambient CO2-reduction as an entry-point for enhanced carbon fixation

Fig. 5: Modules 1, 2 and 4 of the CORE cycle operate robustly together in E. coli.The alternative text for this image may have been generated using AI.

A Selection scheme: Growth of the E. coli C1S-Aux strain is used to detect intracellular formate production via the tested modules of the CORE cycle. For details on formate assimilation (purple arrows), see Fig. 4A. By expressing the C-terminal domain of malonyl-CoA reductase from C. aurantiacus (MCR) together with BKACE from a plasmid (green arrows), endogenous malonyl-CoA is reduced to MSA, providing the substrate for the BKACE reaction. Malonyl-CoA is replenished by the activity of native E. coli acetyl-CoA carboxylase (ACC). B The C1S-Aux strain was tested for growth on different carbon sources in M9 minimal medium with supplementation of glycine (2 mM). Expression of the pathway enzymes (BKACE15 and MCR) rescued growth on different tested carbon sources, such as d-glucose (green line, shortest doubling time (Td) = 4.2 h), l-Lactate (blue, Td = 5.2 h), or glycerol (yellow, Td = 8.9 h). In contrast, no growth is observed with acetoacetate (red) as main carbon source, unless formate (5 mM) is supplemented to rescue the auxotrophy (red, dashed). Additionally, no growth is observed in negative control strains lacking MCR or BKACE15, respectively (shown for d-glucose; gray lines). Shown growth curves represent the mean of technical triplicates, with shaded areas indicating the standard deviation. Source data are provided as a Source Data file.

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